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DNA Structure and Replication

DNA Structure and Replication
How does ethidium bromide interact with DNA?

It does not interact
It binds to adenine
It binds to phosphate
It intercalates between bases

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DNA Structure and Replication
E.coli DNA polymerases II and III lack

5' → 3' exonuclease activity
partially 5' → 3' endonuclease activity
partially 5' → 3' exonuclease activity
5' → 3' endonuclease activity

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DNA Structure and Replication
Which histone(s) are associated with the linker DNA of a nucleosome?

Histone H4
Histone H1
Histones H2A and H2B
Histone H3

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DNA Structure and Replication
The melting of 40-45 bp at the oriC site of E. coli requires

DNA + ATP + HU + DNAb
DNA+ ATP + HU + DNAb + DNAa
DNA + ATP + HU
DNA + ATP + HU + DNAb + DNAa + Pol III holoenzyme

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DNA Structure and Replication
One hypothesis of DNA replication suggested that the parental DNA molecules are broken into fragments. Both strands of DNA in each of the daughter molecules are made up of an assortment of parental and new DNA. This statement refers to which hypothesis?

Conservative theory
Dispersive theory
Evolutionary theory
Semi-conservative theory

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DNA Structure and Replication
When DNA polymerase is in contact with guanine in the parental strand, what does it add to the growing daughter strand?

Guanine
Phosphate
Cytosine
Uracil

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