DNA Structure and Replication
The melting of 40-45 bp at the oriC site of E. coli requires

DNA + ATP + HU
DNA + ATP + HU + DNAb
DNA+ ATP + HU + DNAb + DNAa
DNA + ATP + HU + DNAb + DNAa + Pol III holoenzyme

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DNA Structure and Replication
In DNA, nucleotides are covalently joined together by

3', 4' phosphodiester bonds to form a repetitive sugar-phosphate chain
3', 5' phosphodiester bonds to form a repetitive sugar-phosphate chain
2', 3' phosphodiester bonds to form a repetitive sugar-phosphate chain
2', 5' phosphodiester bonds to form a repetitive sugar-phosphate chain

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