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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
Yeast Replicating plasmids (YRp) carry

autonomously replicating sequence
both (a) and (c)
telomere
centromere

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Vectors Uses for Animal Cell Culture
Which one of the following is true about Yeast Episomal plasmids (YEp) vectors?

Carries autonomously replicating sequence
Carries centromere and telomere sequences
Allows transformation by using crossing over
Has a origin of replication and rep genes

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Vectors Uses for Animal Cell Culture
Which of the following is/are commonly used promoter of S. cerevisiae?

Phosphoglycerate kinase (PGK)
All of these
Galactokinase (GAL 1)
Alcohol dehydrogenase II (ADH 2)

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Vectors Uses for Animal Cell Culture
Which of the following histone is not present in SV40 virus?

H3
H4
H1
H2A

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Vectors Uses for Animal Cell Culture
Which of the following are produced in high copy number?

YRp
YCp
YEp
Both (a) and (b)

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Vectors Uses for Animal Cell Culture
Recombinant SV40 viruses introduce the foreign DNA into cells

without the DNA mediated transfection
without the RNA mediated transfection
with the RNA mediated transfection
with the DNA mediated transfection

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