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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
Which of the following histone is not present in SV40 virus?

H2A
H3
H4
H1

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Vectors Uses for Animal Cell Culture
Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA

integrated only
episomally only
either episomally or integrated at low copy number
either episomally or integrated at high copy number

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Vectors Uses for Animal Cell Culture
5-fluoro orotic acid inhibits the growth if one of the following marker is present in the yeast. That is

Lys 2
Trp 1
His 3
Ura 3

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Vectors Uses for Animal Cell Culture
Which of the following(s) is/are the most commonly used markers in yeast?

Ura 3
Trp 1
His 3
All of these

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Vectors Uses for Animal Cell Culture
Cloning genes in the yeast offer which of following advantage(s)?

All of these
Ability to clone large piece of DNA
Over production of proteins of commercial value
Ability to glycosylate proteins during secretion

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Vectors Uses for Animal Cell Culture
Yeast Replicating plasmids (YRp) carry

telomere
autonomously replicating sequence
centromere
both (a) and (c)

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