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Protein Purification

Protein Purification
Which of the following may be added to stabilize the protein after yeast cells disruption?

NaCl
All of these
Protease inhibitor
AMP

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Protein Purification
Proteins separation can be carried out on the basis of

solubility in salt solutions
all of these
size or mass
net charge

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Protein Purification
Gel-filtration chromatography separates on the basis of

shape using porous beads packed in a column
None of these
size using porous beads packed in a column
size and shape using porous beads packed in a column

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Protein Purification
A purified protein sample contains 10 μg of protein and has an enzyme activity of 1 m mole of ATP synthesized/sec (1 unit). What is the specific activity of the final purified sample?

10,000 units/mg.
1,000 units/mg
100,000 units/mg
1,000,000 units/mg

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Protein Purification
During successful purification scheme, this may be expected that the

both (a) and (c)
specific activity increases
number of proteins in the sample decreases
specific activity decreases

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Protein Purification
The best way to determine the location of protein in the purification scheme is to measure the

UV absorption
mass spectroscopy of the protein
rate of ATP synthesis
changes in the refractive index

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