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Protein Purification

Protein Purification
Which of the following may be added to stabilize the protein after yeast cells disruption?

Protease inhibitor
All of these
AMP
NaCl

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Protein Purification
Proteins separation can be carried out on the basis of

net charge
size or mass
all of these
solubility in salt solutions

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Protein Purification
Affinity chromatography deals with the

protein - carbohydrate interaction
protein - protein interaction
None of these
specific binding of a protein constituents for another molecule

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Protein Purification
During successful purification scheme, this may be expected that the

both (a) and (c)
specific activity increases
specific activity decreases
number of proteins in the sample decreases

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Protein Purification
A purified protein sample contains 10 μg of protein and has an enzyme activity of 1 m mole of ATP synthesized/sec (1 unit). What is the specific activity of the final purified sample?

10,000 units/mg.
1,000 units/mg
100,000 units/mg
1,000,000 units/mg

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Protein Purification
Gel-filtration chromatography separates on the basis of

None of these
shape using porous beads packed in a column
size and shape using porous beads packed in a column
size using porous beads packed in a column

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