Protein Purification A purified protein sample contains 10 μg of protein and has an enzyme activity of 1 m mole of ATP synthesized/sec (1 unit). What is the specific activity of the final purified sample? 10,000 units/mg. 100,000 units/mg 1,000 units/mg 1,000,000 units/mg 10,000 units/mg. 100,000 units/mg 1,000 units/mg 1,000,000 units/mg ANSWER DOWNLOAD EXAMIANS APP
Protein Purification Which of the following may be added to stabilize the protein after yeast cells disruption? NaCl AMP Protease inhibitor All of these NaCl AMP Protease inhibitor All of these ANSWER DOWNLOAD EXAMIANS APP
Protein Purification Gel-filtration chromatography separates on the basis of size using porous beads packed in a column size and shape using porous beads packed in a column shape using porous beads packed in a column None of these size using porous beads packed in a column size and shape using porous beads packed in a column shape using porous beads packed in a column None of these ANSWER DOWNLOAD EXAMIANS APP
Protein Purification During successful purification scheme, this may be expected that the specific activity increases specific activity decreases number of proteins in the sample decreases both (a) and (c) specific activity increases specific activity decreases number of proteins in the sample decreases both (a) and (c) ANSWER DOWNLOAD EXAMIANS APP
Protein Purification In ion-exchange chromatography proteins are separated on the basis of their net charge either (b) or (c) proteins are separated on the basis of their shape proteins are separated on the basis of their size proteins are separated on the basis of their net charge either (b) or (c) proteins are separated on the basis of their shape proteins are separated on the basis of their size ANSWER DOWNLOAD EXAMIANS APP
Protein Purification The best way to determine the location of protein in the purification scheme is to measure the rate of ATP synthesis mass spectroscopy of the protein UV absorption changes in the refractive index rate of ATP synthesis mass spectroscopy of the protein UV absorption changes in the refractive index ANSWER DOWNLOAD EXAMIANS APP
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