Protein Purification A purified protein sample contains 10 μg of protein and has an enzyme activity of 1 m mole of ATP synthesized/sec (1 unit). What is the specific activity of the final purified sample? 10,000 units/mg. 1,000 units/mg 100,000 units/mg 1,000,000 units/mg 10,000 units/mg. 1,000 units/mg 100,000 units/mg 1,000,000 units/mg ANSWER DOWNLOAD EXAMIANS APP
Protein Purification Which of the following may be added to stabilize the protein after yeast cells disruption? NaCl AMP All of these Protease inhibitor NaCl AMP All of these Protease inhibitor ANSWER DOWNLOAD EXAMIANS APP
Protein Purification During successful purification scheme, this may be expected that the both (a) and (c) specific activity increases specific activity decreases number of proteins in the sample decreases both (a) and (c) specific activity increases specific activity decreases number of proteins in the sample decreases ANSWER DOWNLOAD EXAMIANS APP
Protein Purification The best way to determine the location of protein in the purification scheme is to measure the UV absorption mass spectroscopy of the protein rate of ATP synthesis changes in the refractive index UV absorption mass spectroscopy of the protein rate of ATP synthesis changes in the refractive index ANSWER DOWNLOAD EXAMIANS APP
Protein Purification Proteins separation can be carried out on the basis of all of these solubility in salt solutions size or mass net charge all of these solubility in salt solutions size or mass net charge ANSWER DOWNLOAD EXAMIANS APP
Protein Purification In ion-exchange chromatography proteins are separated on the basis of their net charge proteins are separated on the basis of their shape either (b) or (c) proteins are separated on the basis of their size proteins are separated on the basis of their net charge proteins are separated on the basis of their shape either (b) or (c) proteins are separated on the basis of their size ANSWER DOWNLOAD EXAMIANS APP
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