Recombinant DNA Technology
Which of the following is not true of small inbreeding populations?

Alleles may become fixed
Mutation is increased
They tend to loose genetic diversity
There is an increased incidence of recessive diseases

ANSWER DOWNLOAD EXAMIANS APP

Recombinant DNA Technology
The order for the construction of a cDNA fragment from mRNA is to

bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase
digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase
treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT
bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase

ANSWER DOWNLOAD EXAMIANS APP

Recombinant DNA Technology
Which of the following is correct in terms of determination of location of genetic traits?

None of these
Protein-coding genes are always associated with a restriction pattern
Known protein coding sequences are too far apart to allow linkage determination for most new genes
Restriction sites allow DNAs to be digested

ANSWER DOWNLOAD EXAMIANS APP

Recombinant DNA Technology
Knockout mice are created by

infecting the mouse with a retrovirus
mutagenizing a mouse and selecting for mutant offspring
transfecting embryonic stem cells with an altered gene sequence
creating a chimera by fusing cells from two different cell lines

ANSWER DOWNLOAD EXAMIANS APP

Recombinant DNA Technology
An example of a restriction fragment length polymorphism is

an Eco RI cuts DNA at a different sequence than Hind III
All of these
different length fragments of DNA resulting from loss or gain of a restriction site
cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved

ANSWER DOWNLOAD EXAMIANS APP