Immunological Techniques What is the ELISA test intended to measure? Antigen to HIV only Presence of free, circulating virus in the patient Antibodies directed against HLA molecules Antibody to HIV only Antigen to HIV only Presence of free, circulating virus in the patient Antibodies directed against HLA molecules Antibody to HIV only ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight? Open circular, linear plasmid, super coiled plasmid Linear plasmid, open circular, super coiled plasmid Open circular, super coiled, linear plasmid DNA Open circular, super coiled, linear plasmid DNA Open circular, linear plasmid, super coiled plasmid Linear plasmid, open circular, super coiled plasmid Open circular, super coiled, linear plasmid DNA Open circular, super coiled, linear plasmid DNA ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Immunoelectrophoresis techniques are designed to separate the mixture components from each other by using electrophoresis after reaction with antigen prior to reaction with antibody prior to reaction with antigen after reaction with antibody after reaction with antigen prior to reaction with antibody prior to reaction with antigen after reaction with antibody ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added? All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate The ELISA would develop normally None of these The ELISA would not develop when the substrate was added All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate The ELISA would develop normally None of these The ELISA would not develop when the substrate was added ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if serum is omitted from the ELISA, but all other steps remain same? Both (a) and (b) Anti-human Ig-conjugate would bind non-specifically to the ELISA plate Anti-human Ig-conjugate would not bind and be washed away The OD values would be nearly the same as the assay control Both (a) and (b) Anti-human Ig-conjugate would bind non-specifically to the ELISA plate Anti-human Ig-conjugate would not bind and be washed away The OD values would be nearly the same as the assay control ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Agarose gel electrophoresis and pulsed field gel electrophoresis may be used to resolve respectively 1000 kb and l0kb DNA 20 kb and 2000 kb DNA 2000 kb and 20kb DNA 10 kb and 1000 kb DNA 1000 kb and l0kb DNA 20 kb and 2000 kb DNA 2000 kb and 20kb DNA 10 kb and 1000 kb DNA ANSWER DOWNLOAD EXAMIANS APP
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