Immunological Techniques What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added? The ELISA would not develop when the substrate was added None of these The ELISA would develop normally All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate The ELISA would not develop when the substrate was added None of these The ELISA would develop normally All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight? Open circular, linear plasmid, super coiled plasmid Open circular, super coiled, linear plasmid DNA Open circular, super coiled, linear plasmid DNA Linear plasmid, open circular, super coiled plasmid Open circular, linear plasmid, super coiled plasmid Open circular, super coiled, linear plasmid DNA Open circular, super coiled, linear plasmid DNA Linear plasmid, open circular, super coiled plasmid ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques The IPTG is a gratuitous inducers of (β-galactosidase, and x-gal is a chromogenic substrate. When α-complementation occurs which colour is produced? Blue No colour is produced Brown White Blue No colour is produced Brown White ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that the temperature was too high the antibody was impure there was more than one antigen there was one antibody and one antigen the temperature was too high the antibody was impure there was more than one antigen there was one antibody and one antigen ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Which of the following is an immunodiffusion test? Double-diffusion All of these Ouchterloney technique Gel diffusion Double-diffusion All of these Ouchterloney technique Gel diffusion ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What radioactive molecule is used to end-label an oligonucleotide? (gamma)-32P-ATP Any of these (alpha)-32P-ATP (alpha)-32P-dUTP (gamma)-32P-ATP Any of these (alpha)-32P-ATP (alpha)-32P-dUTP ANSWER DOWNLOAD EXAMIANS APP
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