Media Required for Plant Cell Culture
Very high sugar concentration (40-100 g/l) have been used

Both (a) and (b)
to adjust the osmotic potential of the media in short term treatment for regeneration
none of these
in specialized secondary metabolite production

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Media Required for Plant Cell Culture
Nitrogen in the plant cell culture media is provided by either ammonia or nitrate salt. In the media

utilization of ammonium cause culture pH to drop while utilization of nitrate cause culture pH to rise
utilization of both ammonium and nitrate result in rise in pH
utilization of both ammonium and nitrate result in drop in pH
utilization of nitrate cause culture pH to drop while utilization of ammonium cause culture pH to rise

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