Media Required for Plant Cell Culture
Silver thiosulphate is added to culture medium as it helps to

prevent the gaseous plant hormone, ethylene dioxide from accumulating to detrimental condition.
maintain the pH
remove toxic phenolics from plant cells
All of these

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Media Required for Plant Cell Culture
Nitrogen in the plant cell culture media is provided by either ammonia or nitrate salt. In the media

utilization of nitrate cause culture pH to drop while utilization of ammonium cause culture pH to rise
utilization of ammonium cause culture pH to drop while utilization of nitrate cause culture pH to rise
utilization of both ammonium and nitrate result in rise in pH
utilization of both ammonium and nitrate result in drop in pH

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