FT IR Spectroscopy
The sequence of amino acids in proteins can be determined by means of

identification of the -COOH terminal amino acids
identification of the -NH2 terminal amino acids
partial cleavage of the original polypeptide into smaller polypeptides
All of these

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FT IR Spectroscopy
In scanning electron microscopy

None of these
a specimen is fixed and then coated with thin layer of a heavy metal
a specimen is fixed and then coated with transition metal
a specimen is not fixed and then coated with thin layer of a heavy metal

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