FT IR Spectroscopy
Which of the following is the basis of first dimension of separation for two-dimensional electrophoresis?

Folding
Molecular mass
Solubility
Isoelectric point

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FT IR Spectroscopy
Why is it advantageous to record many FID signals from the same sample and then add them together?

To increase sensitivity
None of these
To ensure that all target nuclei in the sample have been excited
To remove inaccuracies caused by fluctuations in the applied magnetic field

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FT IR Spectroscopy
When radiation energy is absorbed by a spin 1/2 nucleus in a magnetic field, what happens?

The nucleus spins faster
The angle of precession flips so that the magnetic moment of the nucleus opposes the applied field
The processional frequency of the nucleus increases
None of these

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FT IR Spectroscopy
In FT-NMR, how are nuclei excited?

By an intense pulse of radiation which contains a wide range of frequencies
None of these
By radio-frequency radiation whose frequency is swept across a predetermined range
By an intense pressure

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