Polymerase Chain Reaction
The PCR/sequencing approach to rRNA is most likely to give information about

the type of energy metabolism the bacterium has
the species to which the bacteria are most closely related
morphology of the bacterium
whether the bacterium is motile

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Polymerase Chain Reaction
The efficiency of amplification in PCR in later cycles is reduced due to

reduction in substrate concentration
all of these
insufficient enzyme and time to synthesize mass quantity of DNA
build up of PCR product which competes with primers for hybrid formation

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