Polymerase Chain Reaction
Which of the following infection(s) can be diagnosed by the use of polymerase chain reaction?

All of these
HIV-1 and HIV-2 viruses
Mycobacterium tuberculosis
Hepatitis B virus

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Polymerase Chain Reaction
The efficiency of amplification in PCR in later cycles is reduced due to

all of these
build up of PCR product which competes with primers for hybrid formation
insufficient enzyme and time to synthesize mass quantity of DNA
reduction in substrate concentration

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