Polymerase Chain Reaction
Which of the following infection(s) can be diagnosed by the use of polymerase chain reaction?

All of these
Hepatitis B virus
HIV-1 and HIV-2 viruses
Mycobacterium tuberculosis

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Polymerase Chain Reaction
The efficiency of amplification in PCR in later cycles is reduced due to

all of these
reduction in substrate concentration
build up of PCR product which competes with primers for hybrid formation
insufficient enzyme and time to synthesize mass quantity of DNA

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