Polymerase Chain Reaction
Which of the following infection(s) can be diagnosed by the use of polymerase chain reaction?

Mycobacterium tuberculosis
HIV-1 and HIV-2 viruses
All of these
Hepatitis B virus

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Polymerase Chain Reaction
The efficiency of amplification in PCR in later cycles is reduced due to

build up of PCR product which competes with primers for hybrid formation
insufficient enzyme and time to synthesize mass quantity of DNA
all of these
reduction in substrate concentration

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