Glycolysis
The enzymes of glycolysis in a eukaryotic cell are located in the

cytosol
mitochondrial matrix
plasma membrane
intermembrane space

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Glycolysis
In glycolysis, ATP is formed by the transfer of a high-energy phosphate from 1,3-bisphosphoglycerate to ADR No such high-energy phosphate donor has ever been isolated in mitochondria because

None of these
no such phosphate donor exists
the techniques for isolating the phosphate donor are not refined enough
the high-energy phosphate donor is very short-lived and difficult to isolate

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