Glycolysis
Which of the following is not a mechanism for altering the flux of metabolites through the rate-determining step of a pathway?

Diffusional coupling between adjacent active sites
Covalent modification of the enzyme
Genetic control of the enzyme concentration
Allosteric control of the enzyme activity

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Glycolysis
In glycolysis, ATP is formed by the transfer of a high-energy phosphate from 1,3-bisphosphoglycerate to ADR No such high-energy phosphate donor has ever been isolated in mitochondria because

the techniques for isolating the phosphate donor are not refined enough
the high-energy phosphate donor is very short-lived and difficult to isolate
no such phosphate donor exists
None of these

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Glycolysis
A kinase is an enzyme that

removes phosphate groups of substrates
removes water from a double bond
uses ATP to add a phosphate group to the substrate
uses NADH to change the oxidation state of the substrate

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