Enzymes
The cleavage specificity of trypsin and chymotrypsin depend in part on the

absence of water in the active site
size, shape, and charge of the active site or specificity pocket
proximity of Ser 195 to the active site or specificity pocket
presence of a low-barrier hydrogen bond in the active site or specificity pocket

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Enzymes
In the enzyme-catalyzed reaction shown below, what will be the effect on substances A, B, C, and D of inactivating the enzyme labeled E2? A ---(E1)---> B ---(E2)---> C ---(E3)--->

A will still be produced, but not B, C, or D
A, B, and C will still be produced, but not D
A and B will still be produced, but not C or D
A, B, C, and D will all still be produced

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Enzymes
Which of the following is false statement with regard to comparison between Serine and HIV proteases?

Both use nucleophilic attack to hydrolyze the peptide bond
Both require water to complete the catalytic cycle
Both forms an acyl-enzyme intermediate
Both show specificity for certain amino acid sequences

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