VECTORS USES FOR ANIMAL CELL CULTURE QUIZ

Retroviruses

Bovine papilloma virus (BVP)

All of these

SV 40 virus

either episomally or integrated at low copy number

episomally only

either episomally or integrated at high copy number

integrated only

is inserted between the polyhedrin promoter and downstream sequences

is inserted downstream of viral origin of replication

is inserted randomly in viral genome

is inserted downstream of polyhedrin promoter

SV 40 helper virus

pBR 322 vector

none of these

COS cell line

Carries autonomously replicating sequence

Allows transformation by using crossing over

Has a origin of replication and rep genes

Carries centromere and telomere sequences

6-8 kb

4-6 kb

10-12 kb

20 kb

they cover a wide host range

All of these

viral gene expression is driven by strong promoters

infection does not lead to cell death, infected cells produce virus over an indefinite period

Carries centromere and telomere sequences

Has a origin of replication and rep genes

Carries autonomously replicating sequence

Allows transformation by using crossing over

Vaccinia virus

Papilloma virus

Baculo virus

Retrovirus

Ds DNA genome

RNA genome

cDNA genome

Ss DNA genome