THERMODYNAMICS AND FREE ENERGY QUIZ

Which of the following amino acids interact with the DNA or RNA backbone (i.e. phosphate)?

Leu and Ala

Cys and Met

Lys and Arg

Ile and Val

TRUE ANSWER : ?
YOUR ANSWER : ?

Reverse transcripase from HIV does not

uses RNA as a template

has a 5' to 3' polymerase activity

has a 3'- 5' exonuclease activity

requires a primer

TRUE ANSWER : ?
YOUR ANSWER : ?

The enzyme that joins DNA cuts is called

DNA phosphorylase

ligase

reverse transcriptase

joinase

TRUE ANSWER : ?
YOUR ANSWER : ?

Cleavage of DNA by EcoRI endonuclease results in the formation of

two 3'-phosphate ends and two 3'-OH ends

two 3'-OH ends and two 5'-phosphate ends

one 3'-OH end and two 5'-phosphate end

one 3'-OH end and one 5'-phosphate end

TRUE ANSWER : ?
YOUR ANSWER : ?

Different DNA polymerases play distinct roles in DNA replication and repair in both prokaryotic and eukaryotic cells. All known DNA polymerases synthesize DNA only in the __________ by the addition of dNTPs to a performed primer strand of DNA.

5' to 3' direction

negative direction

positive direction

3' to 5' direction

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following amino acids can interact with the DNA or RNA nucleotide bases via hydrogen bonding?

Ile and Val

Cys and Met

Asn and Gln

Lys and Leu

TRUE ANSWER : ?
YOUR ANSWER : ?

The type II restriction endonucleases, bind to DNA sites

using non-sequence-dependent backbone interactions

All of these

that are usually palindromic

using H-bonds for specificity

TRUE ANSWER : ?
YOUR ANSWER : ?

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