Dwell volume is defined as the time required for the gradient to reach the column the volume of the column between the point at which solvents are mixed and the beginning of the column the volume of solvent contained in a liquid chromatographic column none of these TRUE ANSWER : ? YOUR ANSWER : ?
Which of the following(s) is/are the advantage of HPLC over traditional LPLC (low-pressure liquid chromatography)? Ideal for ionic species and large molecules All of these Greater sensitivity and reusable columns Sample recovery TRUE ANSWER : ? YOUR ANSWER : ?
An isocratic elution in HPLC is one in which the composition of the solvent changes continuously none of these changes in a series of steps remains constant TRUE ANSWER : ? YOUR ANSWER : ?
A gradient elution in HPLC is one in which the composition of the solvent both (a) and (b) remains constant is changed continuously or in a series of steps None of these TRUE ANSWER : ? YOUR ANSWER : ?
HPLC methods include liquid/liquid (partition) chromatography All of these ion exchange and size exclusion chromatography liquid/solid (adsorption) chromatography TRUE ANSWER : ? YOUR ANSWER : ?
The eluent strength is a measure of solvent absorption energy solvent diffusivity solvent mixing index solvent adsorption energy TRUE ANSWER : ? YOUR ANSWER : ?
An eluotropic series ranks solvents by their relative abilities to displace solutes from a given absorbent ranks column packing material by their relative abilities to retain solutes on the column None of these is a measure of the solvent adsorption energy TRUE ANSWER : ? YOUR ANSWER : ?
Which of the following statements is true for a refractive index detector in HPLC? It can only be used for isocratic elutions none of above It is more sensitive than a UV detector It does not respond to many solutes TRUE ANSWER : ? YOUR ANSWER : ?
In reversed phase HPLC, there is a any of the above non polar solvent/polar column non polar solvent/non-polar column polar solvent/non-polar column TRUE ANSWER : ? YOUR ANSWER : ?
Which can be used as a mobile phase in HPLC applications? Any compound with non-solubility in liquid Any compound with solubility in liquid Any of the above Any compound with limited solubility in liquid TRUE ANSWER : ? YOUR ANSWER : ?