RECOMBINANT DNA QUIZ

Which of the following vector can maintain the largest fragment of foreign DNA?

Cosmid

YAC

Phage

Plasmid

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The advantage of using DNA polymerases from thermophilic organisms in PCR is that

All of these

the DNA polymerases of these bacteria never make mistakes while replicating DNA

the DNA polymerases of these bacteria are much faster than those from other organisms

the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands

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Agrobacterium tumefaciens is

a bacterium that can be used to introduce DNA into plants

a disease in humans that causes loss of sight

a disease in humans that causes loss of weight

a fungi that is used to produce antibiotics in large amounts

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Which type of restriction endonuclease cuts the DNA within the recognition site?

Type II

Type III

Type I

All of the these

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Electroporation is

the application of high voltage pulses

the process of separating charged molecules through a gel maintained in an electric field

the process of multiplication of the cells

the process of combining foreign DNA to an electrically charged vector molecule

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Which of the following is not commonly used as vector?

Fungi

Artificial chromosome

Plasmid

Cosmid

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What is the normal role of restriction endonucleases in bacterial cells?

To produce RNA primers for replication

To degrade invading phage DNA

To degrade the bacterial chromosome into small pieces during replication

All of these

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Enzymes that recognize and cleave specific 4 to 8 base pair sequences of DNA are

DNA gyrase

helicases

DNA ligase

restriction endonucleases

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A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as

polymerase chain reaction

DNA replication

northern blotting

southern blotting

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For gene probes to be useful they must

be labeled in some manner to allow detection

both (a) and (b)

None of these

be large enough to contain gene-specific sequences

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