RECOMBINANT DNA QUIZ

Which of the following vector can maintain the largest fragment of foreign DNA?

Cosmid

Phage

YAC

Plasmid

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Agrobacterium tumefaciens is

a disease in humans that causes loss of sight

a bacterium that can be used to introduce DNA into plants

a fungi that is used to produce antibiotics in large amounts

a disease in humans that causes loss of weight

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Enzymes that recognize and cleave specific 4 to 8 base pair sequences of DNA are

DNA gyrase

helicases

restriction endonucleases

DNA ligase

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A genomic library is

a book that describes how to isolate DNA from a particular organism

a place where the information of the genetic organization of organisms are kept.

a database where the sequence of an organism's genome is stored

a collection of many clones possessing different DNA fragments from the same organisms bound to vectors

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The Southern blotting technique depends on

similarities between the sequences of probe protein and experimental protein

similarities between the sequences of probe RNA and experimental RNA

similarities between the sequences of probe DNA and experimental DNA

the molecular mass of proteins

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Which of the following enzyme is used to covalently bond foreign DNA to a vector plasmid?

DNA ligase

DNA polymerase

Restriction endonuclease

DNA helicase

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What is the normal role of restriction endonucleases in bacterial cells?

To degrade invading phage DNA

All of these

To produce RNA primers for replication

To degrade the bacterial chromosome into small pieces during replication

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A molecular technique in which DNA sequences between two oligonucleotide primers can be amplified is known as

northern blotting

polymerase chain reaction

DNA replication

southern blotting

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In genetic engineering, a chimera is

an enzyme that links DNA molecules

a virus that infects bacteria

a plasmid that contains foreign DNA

a fungi

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Problems in obtaining large amounts of proteins encoded by recombinant genes can often be overcome by using

all of these

expression vectors

YACS

BACS

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