A purified protein sample contains 10 μg of protein and has an enzyme activity of 1 m mole of ATP synthesized/sec (1 unit). What is the specific activity of the final purified sample? 1,000 units/mg 10,000 units/mg. 100,000 units/mg 1,000,000 units/mg TRUE ANSWER : ? YOUR ANSWER : ?
Gel-filtration chromatography separates on the basis of size using porous beads packed in a column None of these shape using porous beads packed in a column size and shape using porous beads packed in a column TRUE ANSWER : ? YOUR ANSWER : ?
In ion-exchange chromatography proteins are separated on the basis of their net charge either (b) or (c) proteins are separated on the basis of their size proteins are separated on the basis of their shape TRUE ANSWER : ? YOUR ANSWER : ?
During successful purification scheme, this may be expected that the number of proteins in the sample decreases specific activity increases specific activity decreases both (a) and (c) TRUE ANSWER : ? YOUR ANSWER : ?
Proteins separation can be carried out on the basis of size or mass net charge all of these solubility in salt solutions TRUE ANSWER : ? YOUR ANSWER : ?
Which of the following may be added to stabilize the protein after yeast cells disruption? Protease inhibitor All of these AMP NaCl TRUE ANSWER : ? YOUR ANSWER : ?
Affinity chromatography deals with the None of these protein - carbohydrate interaction protein - protein interaction specific binding of a protein constituents for another molecule TRUE ANSWER : ? YOUR ANSWER : ?
The best way to determine the location of protein in the purification scheme is to measure the UV absorption rate of ATP synthesis mass spectroscopy of the protein changes in the refractive index TRUE ANSWER : ? YOUR ANSWER : ?