PROTEIN PURIFICATION QUIZ

specific binding of a protein constituents for another molecule

None of these

protein - protein interaction

protein - carbohydrate interaction

net charge

size or mass

all of these

solubility in salt solutions

100,000 units/mg

10,000 units/mg.

1,000 units/mg

1,000,000 units/mg

number of proteins in the sample decreases

specific activity decreases

both (a) and (c)

specific activity increases

changes in the refractive index

mass spectroscopy of the protein

UV absorption

rate of ATP synthesis

None of these

size using porous beads packed in a column

shape using porous beads packed in a column

size and shape using porous beads packed in a column

AMP

NaCl

Protease inhibitor

All of these

proteins are separated on the basis of their size

either (b) or (c)

proteins are separated on the basis of their net charge

proteins are separated on the basis of their shape