PROTEIN AND NUCLEIC ACID INTERACTIONS QUIZ

Which of the following amino acids interact with the DNA or RNA backbone (i.e. phosphate)?

Lys and Arg

Cys and Met

Ile and Val

Leu and Ala

TRUE ANSWER : ?
YOUR ANSWER : ?

Reverse transcripase from HIV does not

requires a primer

uses RNA as a template

has a 3'- 5' exonuclease activity

has a 5' to 3' polymerase activity

TRUE ANSWER : ?
YOUR ANSWER : ?

The type II restriction endonucleases, bind to DNA sites

using H-bonds for specificity

using non-sequence-dependent backbone interactions

All of these

that are usually palindromic

TRUE ANSWER : ?
YOUR ANSWER : ?

Which of the following amino acids can interact with the DNA or RNA nucleotide bases via hydrogen bonding?

Asn and Gln

Lys and Leu

Ile and Val

Cys and Met

TRUE ANSWER : ?
YOUR ANSWER : ?

The enzyme that joins DNA cuts is called

DNA phosphorylase

joinase

reverse transcriptase

ligase

TRUE ANSWER : ?
YOUR ANSWER : ?

Different DNA polymerases play distinct roles in DNA replication and repair in both prokaryotic and eukaryotic cells. All known DNA polymerases synthesize DNA only in the __________ by the addition of dNTPs to a performed primer strand of DNA.

negative direction

3' to 5' direction

5' to 3' direction

positive direction

TRUE ANSWER : ?
YOUR ANSWER : ?

Cleavage of DNA by EcoRI endonuclease results in the formation of

two 3'-OH ends and two 5'-phosphate ends

one 3'-OH end and two 5'-phosphate end

two 3'-phosphate ends and two 3'-OH ends

one 3'-OH end and one 5'-phosphate end

TRUE ANSWER : ?
YOUR ANSWER : ?

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