Affinity chromatography deals with the specific binding of a protein constituents for another molecule None of these protein - carbohydrate interaction protein - protein interaction TRUE ANSWER : ? YOUR ANSWER : ?
Gel-filtration chromatography separates on the basis of size and shape using porous beads packed in a column size using porous beads packed in a column shape using porous beads packed in a column None of these TRUE ANSWER : ? YOUR ANSWER : ?
During successful purification scheme, this may be expected that the both (a) and (c) specific activity decreases specific activity increases number of proteins in the sample decreases TRUE ANSWER : ? YOUR ANSWER : ?
A purified protein sample contains 10 μg of protein and has an enzyme activity of 1 m mole of ATP synthesized/sec (1 unit). What is the specific activity of the final purified sample? 10,000 units/mg. 1,000,000 units/mg 1,000 units/mg 100,000 units/mg TRUE ANSWER : ? YOUR ANSWER : ?
Proteins separation can be carried out on the basis of solubility in salt solutions net charge all of these size or mass TRUE ANSWER : ? YOUR ANSWER : ?
In ion-exchange chromatography either (b) or (c) proteins are separated on the basis of their net charge proteins are separated on the basis of their size proteins are separated on the basis of their shape TRUE ANSWER : ? YOUR ANSWER : ?
The best way to determine the location of protein in the purification scheme is to measure the mass spectroscopy of the protein rate of ATP synthesis UV absorption changes in the refractive index TRUE ANSWER : ? YOUR ANSWER : ?
Which of the following may be added to stabilize the protein after yeast cells disruption? All of these AMP NaCl Protease inhibitor TRUE ANSWER : ? YOUR ANSWER : ?