IMMUNOLOGICAL TECHNIQUES QUIZ

The IPTG is a gratuitous inducers of (β-galactosidase, and x-gal is a chromogenic substrate. When α-complementation occurs which colour is produced?

White

Blue

No colour is produced

Brown

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Radial Immunodiffusion is similar to

gel diffusion

double-diffusion

all of these

Ouchterloney technique

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What radioactive molecule is used to end-label an oligonucleotide?

(alpha)-32P-ATP

(alpha)-32P-dUTP

(gamma)-32P-ATP

Any of these

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In Counter immunoelectrophoresis

electrophoresis will drive the antibody and antigen parallel to each other

electrophoresis will drive the antibody and antigen toward each other

the antibody will migrate towards anode

the antibody will migrate towards cathode

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Immunoaffinity chromatography can be used in biochemical applications to

break down antigen and analyze quantitatively

purify protein antigen

None of these

break down antibody structure

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Which of the following is an immunoelectrophoresis technique?

Coimmunoelectrophoresis

Both (a) and (b)

Two-dimensional immunoelectrophoresis

Counterimmunoelectrophoresis

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An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that

the temperature was too high

there was one antibody and one antigen

there was more than one antigen

the antibody was impure

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Which of the following is a positively charged matrix for ion exchange chromatography?

None of these

CM cellulose

DEAE cellulose

Phosphocellulose

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In Rocket Immunodiffusion the length of the rocket is

inversely proportional to the amount of antigen placed in each well

proportional to the amount of antigen placed in each well

proportional to the amount of antibody placed in each well

inversely proportional to the amount of antibody placed in each well

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What is the ELISA test intended to measure?

Antibodies directed against HLA molecules

Antigen to HIV only

Antibody to HIV only

Presence of free, circulating virus in the patient

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