GENERAL PLANT BIOTECHNOLOGY QUIZ

In a plant tumour cell

different parts of the Ti-plasmid are incorporated

may vary from plant to plant

only a small specific segment of callus T DNA is incorporated

complete Ti plasmid is incorporated in plant nuclear DNA

TRUE ANSWER : ?
YOUR ANSWER : ?

On Ti-plasmid T-region or T-DNA is flanked by a direct repeat of

12 bp

20 bp

25 bp

30 bp

TRUE ANSWER : ?
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Integrated octopine T DNA occurs as

single segment

three segments

two segments

four segments

TRUE ANSWER : ?
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Because of large size of Ti-plasmid, intermediate vectors (IV) are developed in which T DNA has been subcloned into

pBR 322 based plasmid vector

all of these

pRK 2013

pRN 3

TRUE ANSWER : ?
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Crown gall tissue

can be cultivated in vitro in absence of bacteria

both (a) and (b)

shows tumorous properties only in presence of bacteria

retains tumorous properties when cultivated

TRUE ANSWER : ?
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Integrated nopaline T-DNA occurs as

three segments

single segment

two segments

four segments

TRUE ANSWER : ?
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Virulence trait of Agrobacterium tumefaciens is borne on

chromosomal DNA

tumour inducing plasmid DNA

cryptic plasmid DNA

both chromosomal and plasmid DNA

TRUE ANSWER : ?
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Which of the following is not true for microinjection technique that involves transfer of DNA into protoplast?

It is carried out with the help of micromanipulator

The recipient cells are immobilized on artificial support or artificially bound to substarate

All of these

It employs needle with diameter greater than cell diameter

TRUE ANSWER : ?
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Liposomes mediated gene transfer in plants involves

use of polyethylene glycol (PEG)

fusion of liposomes with protoplast

All of these

plasmid DNA enclosed in a lipid bag

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The size of the virulent plasmid of Agrobacterium tumefaciens is

40-80 kb

80-120 kb

140-235 kb

>235 kb

TRUE ANSWER : ?
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