GEL ELECTROPHORESIS QUIZ

smaller proteins enter the beads more readily

both (a) and (b)

large proteins enter the beads more readily

large proteins elute first

All of these

smaller proteins migrate more rapidly through the gel

proteins have the same charge-to-mass ratio

proteins are denatured by the SDS

size

relative content of negatively charged residue only

relative content of positively charged residue only

relative content of positively and negatively charged residue

staining them with the dye

using electron microscope only

none of these

measuring their molecular weight

treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis

treated with a oxidizing agent and then with anionic detergent followed by fractionation by electrophoresis

None of these

fractionated by electrophoresis then treated with an oxidizing agent followed by anionic detergent.

histones migrate to the cathode and myoglobin migrates to the anode

both proteins migrate to the anode

histones migrate to the anode and myoglobin migrates to the cathode

both proteins migrate to the cathode

positively charged side chains

negatively charged side chains

different isoelectric points

molecular weight

net positive charges size

net negative charge

net charge and size

net positive charge

SDS-PAGE electrophoresis

isoelectric focusing

combining information from (a)and (b)

gel filtration chromatography