GEL ELECTROPHORESIS QUIZ

both proteins migrate to the anode

both proteins migrate to the cathode

histones migrate to the anode and myoglobin migrates to the cathode

histones migrate to the cathode and myoglobin migrates to the anode

relative content of positively charged residue only

size

relative content of positively and negatively charged residue

relative content of negatively charged residue only

proteins are denatured by the SDS

proteins have the same charge-to-mass ratio

smaller proteins migrate more rapidly through the gel

All of these

large proteins elute first

both (a) and (b)

smaller proteins enter the beads more readily

large proteins enter the beads more readily

net charge and size

net positive charge

net negative charge

net positive charges size

None of these

treated with a oxidizing agent and then with anionic detergent followed by fractionation by electrophoresis

fractionated by electrophoresis then treated with an oxidizing agent followed by anionic detergent.

treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis

negatively charged side chains

positively charged side chains

different isoelectric points

molecular weight

staining them with the dye

measuring their molecular weight

none of these

using electron microscope only

combining information from (a)and (b)

isoelectric focusing

gel filtration chromatography

SDS-PAGE electrophoresis