GEL ELECTROPHORESIS QUIZ

measuring their molecular weight

using electron microscope only

staining them with the dye

none of these

large proteins enter the beads more readily

smaller proteins enter the beads more readily

both (a) and (b)

large proteins elute first

positively charged side chains

different isoelectric points

negatively charged side chains

molecular weight

SDS-PAGE electrophoresis

combining information from (a)and (b)

isoelectric focusing

gel filtration chromatography

net negative charge

net positive charges size

net charge and size

net positive charge

both proteins migrate to the anode

both proteins migrate to the cathode

histones migrate to the cathode and myoglobin migrates to the anode

histones migrate to the anode and myoglobin migrates to the cathode

relative content of positively charged residue only

size

relative content of positively and negatively charged residue

relative content of negatively charged residue only

treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis

None of these

fractionated by electrophoresis then treated with an oxidizing agent followed by anionic detergent.

treated with a oxidizing agent and then with anionic detergent followed by fractionation by electrophoresis

smaller proteins migrate more rapidly through the gel

proteins have the same charge-to-mass ratio

All of these

proteins are denatured by the SDS