ENZYMES QUIZ

Both use nucleophilic attack to hydrolyze the peptide bond

Both show specificity for certain amino acid sequences

Both require water to complete the catalytic cycle

Both forms an acyl-enzyme intermediate

presence of a low-barrier hydrogen bond in the active site or specificity pocket

proximity of Ser 195 to the active site or specificity pocket

absence of water in the active site

size, shape, and charge of the active site or specificity pocket

neutralize the charge on the other's side chain

function as a proton shuttle

clamp the substrate into the active site

keep the specificity pocket open

Catalyze the reduction of pyruvic acid to acetyl Co A and CO2

retard the reduction of pyruvic acid to acetyl Co A and CO2

Catalyze the oxidation of pyruvic acid to acetyl Co A and CO2

decatalyze the oxidation of pyruvic acid to acetyl Co A and CO2

It cleave after His residues

It cleave after Lys residues

It cleave after Arg residues

None of these

Asparagine

both (a) and (b)

water

Serine

58 kJ/mol

88 kJ/mol

40 kJ/mol

49 kJ/mol

hydrophilic specificity pocket

single reactive serine residue

cluster of reactive serine residues

hydrophobic specificity pocket

It helps to transport carbon dioxide in the blood

It catalyzes the production of hormones that maintain the stomach lining

It is critical for the biosynthesis of DNA

It is a chemical derivative of aspirin

Chymotrypsin

Elastase

all of these

Trypsin