Proteins can be visualized directly in gels by measuring their molecular weight using electron microscope only staining them with the dye none of these TRUE ANSWER : ? YOUR ANSWER : ?
In a native PAGE, proteins are separated on the basis of net positive charges size net negative charge net positive charge net charge and size TRUE ANSWER : ? YOUR ANSWER : ?
Proteins are separated in an SDS-PAGE experiment on the basis of their positively charged side chains different isoelectric points molecular weight negatively charged side chains TRUE ANSWER : ? YOUR ANSWER : ?
In an SDS-PAGE proteins have the same charge-to-mass ratio smaller proteins migrate more rapidly through the gel proteins are denatured by the SDS All of these TRUE ANSWER : ? YOUR ANSWER : ?
In SDS-PAGE, the protein sample is first fractionated by electrophoresis then treated with an oxidizing agent followed by anionic detergent. treated with a oxidizing agent and then with anionic detergent followed by fractionation by electrophoresis treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis None of these TRUE ANSWER : ? YOUR ANSWER : ?
The subunit molecular weight as well as the number of subunits in the quaternary structure can be determined by gel filtration chromatography combining information from (a)and (b) SDS-PAGE electrophoresis isoelectric focusing TRUE ANSWER : ? YOUR ANSWER : ?
In a gel filtration column smaller proteins enter the beads more readily large proteins enter the beads more readily both (a) and (b) large proteins elute first TRUE ANSWER : ? YOUR ANSWER : ?
Electrophoresis of histones and myoglobin under non-denaturing conditions (pH = 7.0) results in both proteins migrate to the anode histones migrate to the cathode and myoglobin migrates to the anode both proteins migrate to the cathode histones migrate to the anode and myoglobin migrates to the cathode TRUE ANSWER : ? YOUR ANSWER : ?
In isoelectric focusing, proteins are separated on the basis of their relative content of positively and negatively charged residue size relative content of negatively charged residue only relative content of positively charged residue only TRUE ANSWER : ? YOUR ANSWER : ?