Which of the following(s) is/are the advantage of HPLC over traditional LPLC (low-pressure liquid chromatography)? Greater sensitivity and reusable columns Ideal for ionic species and large molecules Sample recovery All of these TRUE ANSWER : ? YOUR ANSWER : ?
Dwell volume is defined as the time required for the gradient to reach the column the volume of the column between the point at which solvents are mixed and the beginning of the column the volume of solvent contained in a liquid chromatographic column none of these TRUE ANSWER : ? YOUR ANSWER : ?
For a typical adsorbent such as silica gel, the most popular pore diameters are 60 and 100 A° 150 and 200 A° 10 and 50 A° 100 and 150 A° TRUE ANSWER : ? YOUR ANSWER : ?
In normal phase HPLC, there is a non polar solvent/polar column polar solvent/non-polar column any of the above non polar solvent/non-polar column TRUE ANSWER : ? YOUR ANSWER : ?
An eluotropic series ranks column packing material by their relative abilities to retain solutes on the column None of these ranks solvents by their relative abilities to displace solutes from a given absorbent is a measure of the solvent adsorption energy TRUE ANSWER : ? YOUR ANSWER : ?
In reversed phase HPLC, there is a polar solvent/non-polar column non polar solvent/non-polar column non polar solvent/polar column any of the above TRUE ANSWER : ? YOUR ANSWER : ?
Which of the following statements is true for a refractive index detector in HPLC? It is more sensitive than a UV detector It can only be used for isocratic elutions none of above It does not respond to many solutes TRUE ANSWER : ? YOUR ANSWER : ?
The eluent strength is a measure of solvent absorption energy solvent diffusivity solvent adsorption energy solvent mixing index TRUE ANSWER : ? YOUR ANSWER : ?
Column efficiency is measured in terms of number of plates which is directly related to the square of the peak width directly related to the square of the peak width inversely related to the square of the peak width inversely related to the cube root of the peak width TRUE ANSWER : ? YOUR ANSWER : ?
Void volume refers to the total volume of eluent in the column the remainder being taken up by the packing material the time required for the gradient to reach the column the volume of solvent contained in a liquid chromatographic column the volume of the column between the point at which solvents are mixed and the beginning of the column TRUE ANSWER : ? YOUR ANSWER : ?