In the enzyme-catalyzed reaction shown below, what will be the effect on substances A, B, C, and D of inactivating the enzyme labeled E2? A ---(E1)---> B ---(E2)---> C ---(E3)---> A, B, and C will still be produced, but not D A, B, C, and D will all still be produced A will still be produced, but not B, C, or D A and B will still be produced, but not C or D TRUE ANSWER : ? YOUR ANSWER : ?
The E.coli pyruvic acid dehydrogenase complex is reported to decatalyze the oxidation of pyruvic acid to acetyl Co A and CO2 retard the reduction of pyruvic acid to acetyl Co A and CO2 Catalyze the reduction of pyruvic acid to acetyl Co A and CO2 Catalyze the oxidation of pyruvic acid to acetyl Co A and CO2 TRUE ANSWER : ? YOUR ANSWER : ?
Which of the following (s) is/are serine proteases? Elastase all of these Chymotrypsin Trypsin TRUE ANSWER : ? YOUR ANSWER : ?
Before they can react, many molecules need to be destabilized. This state is typically achieved through the input of a small amount of activation energy changing the three-dimensional shape of the molecule changing the reaction from a biosynthetic to a catabolic pathway oxidizing the molecules by removing electrons TRUE ANSWER : ? YOUR ANSWER : ?
The role of Asp 102 and His 57 during trypsin catalysis is to clamp the substrate into the active site keep the specificity pocket open neutralize the charge on the other's side chain function as a proton shuttle TRUE ANSWER : ? YOUR ANSWER : ?
The nucleophile in serine proteases is Serine Asparagine water both (a) and (b) TRUE ANSWER : ? YOUR ANSWER : ?
The cleavage specificity of trypsin and chymotrypsin depend in part on the absence of water in the active site proximity of Ser 195 to the active site or specificity pocket size, shape, and charge of the active site or specificity pocket presence of a low-barrier hydrogen bond in the active site or specificity pocket TRUE ANSWER : ? YOUR ANSWER : ?
The proteolysis rate enhancement by chymotrypsin (~1010 folds) corresponds to a reduction in activation energy of about 40 kJ/mol 58 kJ/mol 88 kJ/mol 49 kJ/mol TRUE ANSWER : ? YOUR ANSWER : ?
Common feature in all serine proteases is a hydrophilic specificity pocket hydrophobic specificity pocket single reactive serine residue cluster of reactive serine residues TRUE ANSWER : ? YOUR ANSWER : ?
Tryprotophan synthetase of E.coli, a typical bifunctional oligomeric enzyme consist of a protein A and one-subunit a a protein designated B a protein designated A two proteins designated A and B TRUE ANSWER : ? YOUR ANSWER : ?