CLONING VECTORS QUIZ

cosmid

phage

plasmid

phagemid

May be propagated as a plasmid or as phage in appropriate strain

Contain functional origin of replication of the plasmid and λ phage

Can only be propagated as phage

Contain λ att site

origin of replication

cleavage site for the insertion of foreign DNA

marker genes coding for replication

genes coding for viral proteins

for oligonucleotide directed mutagenesis

for probe preparation

for sequencing of cloned DNA

All of these

35 kb

27 kb

50 kb

18 kb

do not exhibit any phenotypic trait

exhibit many phenotypic traits

exhibit one phenotypic traits

exhibit antibiotic resistance

contains cleavage site

helps whole genome in circularization and ligation

consists of 12 bases

both (a) and (b)

1-2 kb

6-7 kb

30-35 kb

9-20 kb

40-44 kb

1-2 kb

20-23 kb

6-7 kb

Ampr

Tetr

Both (a) and (b)

Kanr