In isoelectric focusing, proteins are separated on the basis of their relative content of positively charged residue only relative content of positively and negatively charged residue size relative content of negatively charged residue only TRUE ANSWER : ? YOUR ANSWER : ?
The subunit molecular weight as well as the number of subunits in the quaternary structure can be determined by combining information from (a)and (b) isoelectric focusing gel filtration chromatography SDS-PAGE electrophoresis TRUE ANSWER : ? YOUR ANSWER : ?
In an SDS-PAGE proteins are denatured by the SDS All of these proteins have the same charge-to-mass ratio smaller proteins migrate more rapidly through the gel TRUE ANSWER : ? YOUR ANSWER : ?
In a gel filtration column smaller proteins enter the beads more readily both (a) and (b) large proteins elute first large proteins enter the beads more readily TRUE ANSWER : ? YOUR ANSWER : ?
Electrophoresis of histones and myoglobin under non-denaturing conditions (pH = 7.0) results in both proteins migrate to the cathode histones migrate to the anode and myoglobin migrates to the cathode both proteins migrate to the anode histones migrate to the cathode and myoglobin migrates to the anode TRUE ANSWER : ? YOUR ANSWER : ?
In a native PAGE, proteins are separated on the basis of net positive charges size net negative charge net positive charge net charge and size TRUE ANSWER : ? YOUR ANSWER : ?
Proteins can be visualized directly in gels by using electron microscope only staining them with the dye measuring their molecular weight none of these TRUE ANSWER : ? YOUR ANSWER : ?
Proteins are separated in an SDS-PAGE experiment on the basis of their molecular weight positively charged side chains different isoelectric points negatively charged side chains TRUE ANSWER : ? YOUR ANSWER : ?
In SDS-PAGE, the protein sample is first treated with a oxidizing agent and then with anionic detergent followed by fractionation by electrophoresis treated with a reducing agent and then with anionic detergent followed by fractionation by electrophoresis None of these fractionated by electrophoresis then treated with an oxidizing agent followed by anionic detergent. TRUE ANSWER : ? YOUR ANSWER : ?