In animal cell culture, particularly mammalian cell culture, transformation means both (a) and (b) phenotypic modifications of cells in culture release of genetic information uptake of new genetic material TRUE ANSWER : ? YOUR ANSWER : ?
During the growth of animal cells in culture, it is noticed that the cells do not look very healthy. After an investigation, this is found that there is a lot of lactic acid in the culture fluid. What is probably wrong with this culture? Ethyl alcohol is being produced in excess Glycolysis is being inhibited The cells do not have enough oxygen The cells have too much oxygen TRUE ANSWER : ? YOUR ANSWER : ?
Specific oxygen consumption rates for mammalian cells are in the range of (where n and p represents to nano and pico respectively) 0.05-5 pmol of O2/cell/h 0.05-5 nmol of O2/cell/h 5 - 10 nmol of O2 /cell/h 5 - 10 pmol of O2/cell/h TRUE ANSWER : ? YOUR ANSWER : ?
Which of the following is the technique used for the embryo culture? All of these Organ cultures on agar Organ cultures on plasma clots Whole embryo cultures TRUE ANSWER : ? YOUR ANSWER : ?
Toxicity due to accumulation of ammonia can be overcome All of these by removal of ammonia or ammonium from culture medium by substituting glutamine by glutamate by controlled addition of glutamine at low level TRUE ANSWER : ? YOUR ANSWER : ?
Disaggregating of cells can be achieved by treating with chelating agents All of these enzymatic digestion physical disruption TRUE ANSWER : ? YOUR ANSWER : ?
To prevent the accumulation of lactate low glucose concentration is required low glutamine concentration is required high glucose concentration is required high glutamine concentration is required TRUE ANSWER : ? YOUR ANSWER : ?
How does CO2 help in the cell metabolism during cell culture? It participates in the de novo synthesis of purines and pyrimidines All of these For monitoring pH of the culture Helps in the cells respiration TRUE ANSWER : ? YOUR ANSWER : ?
At low glucose concentration, below 0.25 mmol/litre, large portions of glucose and glutamine is shunted via anaerobic pathway oxidative pathway both (a) and (b) none of these TRUE ANSWER : ? YOUR ANSWER : ?
pH of culture medium is initially controlled by addition of bases none of these presence of bicarbonate buffer presence of CO2 TRUE ANSWER : ? YOUR ANSWER : ?