ANIMAL CELL CULTURE AND REGULATION QUIZ

increase in specific lactate production from glucose

incomplete glutamine oxidation

accumulation of ammonia

both (a) and (b)

5.5 - 55 mmol/litre

75-105 mmol/litre

55 - 75 mmol/litre

105-150 mmol/litre

Organ cultures on plasma clots

Organ cultures on agar

Whole embryo cultures

All of these

by removal of ammonia or ammonium from culture medium

by substituting glutamine by glutamate

All of these

by controlled addition of glutamine at low level

ATP

Creatine phosphate

Lactic acid

Glucose

The cells do not have enough oxygen

Glycolysis is being inhibited

Ethyl alcohol is being produced in excess

The cells have too much oxygen

oxidative pathway

anaerobic pathway

none of these

both (a) and (b)

lipid peroxidation

All of these

DNA degradation

metabolism of nutrients in culture media at a rate greater than that required for consumption

40 times at an interval of 3 days between subcultures

50 times at an interval of 3 days between subcultures

70 times at an interval of 1 day between subcultures

70 times at an interval of 3 days between subcultures

both (a) and (b)

semi-solid medium

liquid medium

solid medium