ANIMAL BREEDING AND TRANSGENIC ANIMAL QUIZ

Opines that are present in crown gall tumour include

nopaline

all of these

agropine

octopine

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Agrobacterium tumefaciens is a

a fungi

gram (-) bacteria

gram (+) bacteria

a yeast

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The transfer of intermediate vectors into Agrobacterium are brought about by

transduction

transformation

triparental mating

biparental mating

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The direct repeats flanking the T-DNA of Agrobacterium tume-faciens are known as

flanking sequences

border sequences

cos site

transfer sequences

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In the liposome mediated gene transfer in plants, nucleic acids are

protected from nuclease digestion

not stable in liposomes

both (a) and (b)

stable in liposomes

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In a plant tumour cell

complete Ti plasmid is incorporated in plant nuclear DNA

different parts of the Ti-plasmid are incorporated

may vary from plant to plant

only a small specific segment of callus T DNA is incorporated

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Opines are

amino acid derivatives found in tumor tissues

amino acid derivatives found in both normal as well as tumor tissues

amino acid derivatives found in normal tissues

None of these

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Liposomes mediated gene transfer in plants involves

All of these

use of polyethylene glycol (PEG)

fusion of liposomes with protoplast

plasmid DNA enclosed in a lipid bag

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The right segment of octopine T-DNA (TR) is necessary for

enzymes for agropine biosynthesis

conjugative transfer

tumour formation

all of these

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The size of the virulent plasmid of Agrobacterium tumefaciens is

>235 kb

40-80 kb

140-235 kb

80-120 kb

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