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DNA Structure and Replication

DNA Structure and Replication
Enzymes, responsible for unraveling short segments of DNA is

DNA ligase
helicase
DNA polymerase
primase

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DNA Structure and Replication
The RNA primers used to initiate replication in E. coli

are removed by helicase + ATP
are removed by Pol I
are joined together by DNA ligase
result in Okazaki fragments on leading strand

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DNA Structure and Replication
The role of primase is to

dismantle RNA primer
cleave and unwinds short sections of DNA ahead of the replication fork
synthesize an RNA primer to begin the elongation process
proofread base pairing

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DNA Structure and Replication
DNA gyrase in E. coli

adds positive supercoils to chromosomal DNA
can be inhibited with antibiotics
performs the same function as helicase in eukaryotes
is required only at the oriC site

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DNA Structure and Replication
Which is the largest among the followings?

Nucleotide
Carbon
Phosphate
Nitrogenous base

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DNA Structure and Replication
What does ligase do during replication of DNA?

Makes copies of mRNA from DNA
Digests mRNA when it is no longer needed
Joins nicks in DNA strands
Synthesizes the lagging strand

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