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DNA Structure and Replication

DNA Structure and Replication
Enzymes, responsible for unraveling short segments of DNA is

DNA polymerase
helicase
DNA ligase
primase

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DNA Structure and Replication
The RNA primers used to initiate replication in E. coli

are removed by Pol I
are removed by helicase + ATP
are joined together by DNA ligase
result in Okazaki fragments on leading strand

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DNA Structure and Replication
DNA with a G-C content of 50% will melt at approximately

60°C
100 °C
70 °C
90 °C

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DNA Structure and Replication
What is the range of melting point temperatures (Tm) for most DNA molecules?

70 to 90°C
60 to 80°C
50 to 60°C
80 to l00°C

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DNA Structure and Replication
Okazaki fragments occur on the __________ and are bonded together by __________

Lagging strand, ligase
Leading strand, polymerase
mRNA, anticodons
tRNA, polymerase

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DNA Structure and Replication
Replication in E. coli is initiated by the generation of short RNA primers using

RNA polymerase
reverse transcriptase
both (a) and (b)
primase

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