Vectors Uses for Animal Cell Culture
Cos cell line is

derivative of non-permissive CV-1 monkey cell line
derivative of permissive CV-1 monkey cell line
cohesive initial site of phage λ
cohesive end site of phage λ

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Vectors Uses for Animal Cell Culture
Recombinant adeno virus can be created by deleting E1A/E 1B genes that are

coding for virus coat protein
not necessary for viral replication
transcriptional regulators and are essential for viral replication
important for packaging of viral DNA into viral head

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