Vectors Uses for Animal Cell Culture
Cloning genes in the yeast offer which of following advantage(s)?

Over production of proteins of commercial value
Ability to glycosylate proteins during secretion
All of these
Ability to clone large piece of DNA

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Vectors Uses for Animal Cell Culture
Yeast episomal plasmids (YEp) were constructed using

2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322
2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9
12μm yeast plasmid and E coli vector pMB 9
12μm yeast plasmid and E coli vector pBR 322

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Vectors Uses for Animal Cell Culture
In case of Baculoviruses, the foreign gene

is inserted between the polyhedrin promoter and downstream sequences
is inserted randomly in viral genome
is inserted downstream of polyhedrin promoter
is inserted downstream of viral origin of replication

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Vectors Uses for Animal Cell Culture
Recombinant adeno virus can be created by deleting E1A/E 1B genes that are

important for packaging of viral DNA into viral head
not necessary for viral replication
transcriptional regulators and are essential for viral replication
coding for virus coat protein

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