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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
5-fluoro orotic acid inhibits the growth if one of the following marker is present in the yeast. That is

His 3
Trp 1
Ura 3
Lys 2

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Vectors Uses for Animal Cell Culture
Lytic infection of SV 40 does not occur in

none of these
both (a) and (b)
non permissive cells
permissive cells

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Vectors Uses for Animal Cell Culture
Which of the following are produced in high copy number?

YEp
YCp
YRp
Both (a) and (b)

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Vectors Uses for Animal Cell Culture
SV 40 vectors, in which late region replacements are done, require

none of these
COS cell line
SV 40 helper virus
pBR 322 vector

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Vectors Uses for Animal Cell Culture
Which one of the following is the most stable vector?

YEp
YRp
YAC
YIp

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Vectors Uses for Animal Cell Culture
Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA

either episomally or integrated at high copy number
either episomally or integrated at low copy number
episomally only
integrated only

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