DNA Structure and Replication Which of the following is used to determine the appropriate stringency (Wallace rule)? Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair) Tm = 4 x (No.of A:T base pair) + 2 x (No.of G:C base pair) Tm = 69.3° + 0.41 (%)G+C -650/1 Tm = 2 x (No.of G:C base pair) + 4 x (No.of A:T base pair) Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair) Tm = 4 x (No.of A:T base pair) + 2 x (No.of G:C base pair) Tm = 69.3° + 0.41 (%)G+C -650/1 Tm = 2 x (No.of G:C base pair) + 4 x (No.of A:T base pair) ANSWER DOWNLOAD EXAMIANS APP
DNA Structure and Replication In the study of one experiment it was found that the value of Tm for DNA is = 40° C. If the cell has 20% GC at the above Tm, then what will be value of 'Tm' if the GC% increases to 60%? Can not be compared Remains same Decreases Increases Can not be compared Remains same Decreases Increases ANSWER DOWNLOAD EXAMIANS APP
DNA Structure and Replication The role of primase is to synthesize an RNA primer to begin the elongation process proofread base pairing cleave and unwinds short sections of DNA ahead of the replication fork dismantle RNA primer synthesize an RNA primer to begin the elongation process proofread base pairing cleave and unwinds short sections of DNA ahead of the replication fork dismantle RNA primer ANSWER DOWNLOAD EXAMIANS APP
DNA Structure and Replication Which of the following is an active site on its single polypeptide chain for DNA Polymerase I? 5' → 3' exonuclease All of these 5' → 3' polymerase 3' → 5' exonuclease 5' → 3' exonuclease All of these 5' → 3' polymerase 3' → 5' exonuclease ANSWER DOWNLOAD EXAMIANS APP
DNA Structure and Replication TM refers to the temperature at which membranes are 50% fluid All of these 50% of a protein molecules are denatured 50% of a DNA molecule is denatured membranes are 50% fluid All of these 50% of a protein molecules are denatured 50% of a DNA molecule is denatured ANSWER DOWNLOAD EXAMIANS APP
DNA Structure and Replication The melting of 40-45 bp at the oriC site of E. coli requires DNA + ATP + HU DNA+ ATP + HU + DNAb + DNAa DNA + ATP + HU + DNAb + DNAa + Pol III holoenzyme DNA + ATP + HU + DNAb DNA + ATP + HU DNA+ ATP + HU + DNAb + DNAa DNA + ATP + HU + DNAb + DNAa + Pol III holoenzyme DNA + ATP + HU + DNAb ANSWER DOWNLOAD EXAMIANS APP
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