Immunological Techniques What radioactive molecule is used to end-label an oligonucleotide? Any of these (alpha)-32P-ATP (alpha)-32P-dUTP (gamma)-32P-ATP Any of these (alpha)-32P-ATP (alpha)-32P-dUTP (gamma)-32P-ATP ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight? Linear plasmid, open circular, super coiled plasmid Open circular, super coiled, linear plasmid DNA Open circular, linear plasmid, super coiled plasmid Open circular, super coiled, linear plasmid DNA Linear plasmid, open circular, super coiled plasmid Open circular, super coiled, linear plasmid DNA Open circular, linear plasmid, super coiled plasmid Open circular, super coiled, linear plasmid DNA ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques Immunoelectrophoresis techniques are designed to separate the mixture components from each other by using electrophoresis after reaction with antigen prior to reaction with antibody after reaction with antibody prior to reaction with antigen after reaction with antigen prior to reaction with antibody after reaction with antibody prior to reaction with antigen ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques What would happen if serum is omitted from the ELISA, but all other steps remain same? Both (a) and (b) Anti-human Ig-conjugate would bind non-specifically to the ELISA plate The OD values would be nearly the same as the assay control Anti-human Ig-conjugate would not bind and be washed away Both (a) and (b) Anti-human Ig-conjugate would bind non-specifically to the ELISA plate The OD values would be nearly the same as the assay control Anti-human Ig-conjugate would not bind and be washed away ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that there was one antibody and one antigen there was more than one antigen the antibody was impure the temperature was too high there was one antibody and one antigen there was more than one antigen the antibody was impure the temperature was too high ANSWER DOWNLOAD EXAMIANS APP
Immunological Techniques The IPTG is a gratuitous inducers of (β-galactosidase, and x-gal is a chromogenic substrate. When α-complementation occurs which colour is produced? No colour is produced Blue White Brown No colour is produced Blue White Brown ANSWER DOWNLOAD EXAMIANS APP
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