Gel Electrophoresis
The subunit molecular weight as well as the number of subunits in the quaternary structure can be determined by

SDS-PAGE electrophoresis
combining information from (a)and (b)
isoelectric focusing
gel filtration chromatography

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Gel Electrophoresis
In an SDS-PAGE

All of these
proteins have the same charge-to-mass ratio
proteins are denatured by the SDS
smaller proteins migrate more rapidly through the gel

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Gel Electrophoresis
Electrophoresis of histones and myoglobin under non-denaturing conditions (pH = 7.0) results in

histones migrate to the anode and myoglobin migrates to the cathode
both proteins migrate to the anode
both proteins migrate to the cathode
histones migrate to the cathode and myoglobin migrates to the anode

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