DNA Structure and Replication
The size of a linear DNA fragment in a gel can be estimated by

the sedimentation coefficient
fluorescence in UV light
comparison with a molecular weight standard such as Hind III digested phage lambda
the time it takes the gel to run

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DNA Structure and Replication
In DNA, nucleotides are covalently joined together by

3', 4' phosphodiester bonds to form a repetitive sugar-phosphate chain
2', 3' phosphodiester bonds to form a repetitive sugar-phosphate chain
3', 5' phosphodiester bonds to form a repetitive sugar-phosphate chain
2', 5' phosphodiester bonds to form a repetitive sugar-phosphate chain

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