Mass Transfer
The recovery of pencillin from the acidified fermentation broth is done by

Evaporation
Distillation
Absorption
Liquid extraction

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Mass Transfer
Fenske's equation for determining the minimum number of theoretical stages in distillation column holds good, when the

Mixture (to be separated) shows negative deviation from ideality
Multicomponent distillation is involved
Relative volatility is reasonably constant
Mixture (to be separated) shows positive deviation from ideality

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