Recombinant DNA
The advantage of using DNA polymerases from thermophilic organisms in PCR is that

the DNA polymerases of these bacteria never make mistakes while replicating DNA
All of these
the DNA polymerases of these bacteria can withstand the high temperatures needed to denature the DNA strands
the DNA polymerases of these bacteria are much faster than those from other organisms

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Recombinant DNA
The Southern blotting technique depends on

similarities between the sequences of probe DNA and experimental DNA
similarities between the sequences of probe RNA and experimental RNA
similarities between the sequences of probe protein and experimental protein
the molecular mass of proteins

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