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Protein and Nucleic Acid Interactions

Protein and Nucleic Acid Interactions
Reverse transcripase from HIV does not

has a 3'- 5' exonuclease activity
requires a primer
uses RNA as a template
has a 5' to 3' polymerase activity

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Protein and Nucleic Acid Interactions
Which of the following amino acids interact with the DNA or RNA backbone (i.e. phosphate)?

Ile and Val
Lys and Arg
Cys and Met
Leu and Ala

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Protein and Nucleic Acid Interactions
The enzyme that joins DNA cuts is called

DNA phosphorylase
reverse transcriptase
joinase
ligase

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Protein and Nucleic Acid Interactions
The type II restriction endonucleases, bind to DNA sites

using non-sequence-dependent backbone interactions
All of these
using H-bonds for specificity
that are usually palindromic

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Protein and Nucleic Acid Interactions
Cleavage of DNA by EcoRI endonuclease results in the formation of

two 3'-phosphate ends and two 3'-OH ends
one 3'-OH end and two 5'-phosphate end
two 3'-OH ends and two 5'-phosphate ends
one 3'-OH end and one 5'-phosphate end

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Protein and Nucleic Acid Interactions
Which of the following amino acids can interact with the DNA or RNA nucleotide bases via hydrogen bonding?

Asn and Gln
Cys and Met
Ile and Val
Lys and Leu

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