Plant Cell Culture
Protoplasts can be produced from suspension cultures, callus tissues or intact tissues by enzymatic treatment with

both cellulotyic and pectolytic enzymes
proteolytic enzymes
cellulotyic enzymes
pectolytic enzymes

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Plant Cell Culture
What is meant by 'Organ culture' ?

Maintenance alive of a whole organ, after removal from the organism by partial immersion in a nutrient fluid
The aspects of culture in community which are mainly dedicated by the need of a specified organ of the human body
Introduction of a new organ in an animal body with a view to create genetic mutation in the progenies of that animal
Cultivation of organs in a laboratory through the synthesis of tissues

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