Plant Cell Culture
Protoplasts can be produced from suspension cultures, callus tissues or intact tissues by enzymatic treatment with

pectolytic enzymes
proteolytic enzymes
cellulotyic enzymes
both cellulotyic and pectolytic enzymes

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Plant Cell Culture
In a callus culture

increasing level of auxin to a callus induces shoot formation and increasing level of cytokinin promote root formation
auxins and cytokinins are not required
only auxin is required for root and shoot formation
increasing level of cytokinin to a callus induces shoot formation and increasing level of auxin promote root formation

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