Microbial Recombination and Gene Transfer
When composite transposons are formed

two IS elements integrate into a chromosome with only a small distance separating them
a small deletion occurs in the transposase gene of an IS element and plasmid is integrated
a small deletion occurs in the transposase gene of an IS element
an IS element integrates with another IS element with the help of a plasmid

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Microbial Recombination and Gene Transfer
The main difference between a self-transmissible and a mobilizable plasmid is that the self-transmissible plasmid

transfers both strands of the plasmid DNA
carries genes encoding the mating apparatus
transfers antibiotic resistance genes
usually has a transposon inserted into it

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Microbial Recombination and Gene Transfer
The expression of gene X (which has promoter Px) is to be monitored. A gene fusion construction for carrying this work will

allow to monitor the expression of all genes with a promoter similar in sequence to Px
have the promoter of lacZ or some other reporter gene
give the same information as from a microarray
have Px but not the rest of the X coding region

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