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Cloning Vectors

Cloning Vectors
pBR 322 has/have which of the following selection marker(s)?

Ampr
Tetr
Kanr
Both (a) and (b)

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Cloning Vectors
Plasmids which are maintained as limited number of copies per cell are known as

stringent plasmids
all of these
relaxed plasmids
cryptic plasmids

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Cloning Vectors
Size of the DNA that can be packaged into a λ phage is

any size
50 kb
40-50 kb
35-53 kb

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Cloning Vectors
A plasmid can be considered as a suitable cloning vector if

insertion of foreign DNA does not alter its replication properties
All of these
it can be readily isolated from the cells
it possesses a single restriction site for one or more restriction enzymes

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Cloning Vectors
Single stranded vectors are useful

All of these
for sequencing of cloned DNA
for probe preparation
for oligonucleotide directed mutagenesis

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Cloning Vectors
Cryptic plasmids

do not exhibit any phenotypic trait
exhibit one phenotypic traits
exhibit antibiotic resistance
exhibit many phenotypic traits

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